The route of inoculation determines the tissue tropism of modified vaccinia Tiantan expressing the spike glycoprotein of SARS-CoV in mice.
Identifieur interne : 002417 ( Main/Exploration ); précédent : 002416; suivant : 002418The route of inoculation determines the tissue tropism of modified vaccinia Tiantan expressing the spike glycoprotein of SARS-CoV in mice.
Auteurs : Huan Liu [République populaire de Chine] ; Wenbo Yu ; Xian Tang ; Haibo Wang ; Wenjie Ouyang ; Jingying Zhou ; Zhiwei ChenSource :
- Journal of medical virology [ 1096-9071 ] ; 2010.
Descripteurs français
- KwdFr :
- Administration par voie nasale, Administration par voie vaginale, Animaux, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Hybridation in situ, Immunohistochimie, Injections musculaires, Protéines de l'enveloppe virale (génétique), Réaction de polymérisation en chaîne, Souris, Souris de lignée BALB C, Structures anatomiques de l'animal (anatomopathologie), Structures anatomiques de l'animal (virologie), Tropisme viral, Vaccins antiviraux (administration et posologie), Vaccins antiviraux (effets indésirables), Vaccins atténués (administration et posologie), Vaccins atténués (effets indésirables), Virus de la vaccine (génétique), Virus de la vaccine (physiologie), Virus du SRAS (génétique).
- MESH :
- administration et posologie : Vaccins antiviraux, Vaccins atténués.
- anatomopathologie : Structures anatomiques de l'animal.
- effets indésirables : Vaccins antiviraux, Vaccins atténués.
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus de la vaccine, Virus du SRAS.
- physiologie : Virus de la vaccine.
- virologie : Structures anatomiques de l'animal.
- Administration par voie nasale, Administration par voie vaginale, Animaux, Glycoprotéine de spicule des coronavirus, Hybridation in situ, Immunohistochimie, Injections musculaires, Réaction de polymérisation en chaîne, Souris, Souris de lignée BALB C, Tropisme viral.
English descriptors
- KwdEn :
- Administration, Intranasal, Administration, Intravaginal, Animal Structures (pathology), Animal Structures (virology), Animals, Immunohistochemistry, In Situ Hybridization, Injections, Intramuscular, Membrane Glycoproteins (genetics), Mice, Mice, Inbred BALB C, Polymerase Chain Reaction, SARS Virus (genetics), Spike Glycoprotein, Coronavirus, Vaccines, Attenuated (administration & dosage), Vaccines, Attenuated (adverse effects), Vaccinia virus (genetics), Vaccinia virus (physiology), Viral Envelope Proteins (genetics), Viral Tropism, Viral Vaccines (administration & dosage), Viral Vaccines (adverse effects).
- MESH :
- chemical , administration & dosage : Vaccines, Attenuated, Viral Vaccines.
- chemical , adverse effects : Vaccines, Attenuated, Viral Vaccines.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : SARS Virus, Vaccinia virus.
- pathology : Animal Structures.
- physiology : Vaccinia virus.
- virology : Animal Structures.
- Administration, Intranasal, Administration, Intravaginal, Animals, Immunohistochemistry, In Situ Hybridization, Injections, Intramuscular, Mice, Mice, Inbred BALB C, Polymerase Chain Reaction, Spike Glycoprotein, Coronavirus, Viral Tropism.
Abstract
The live replication-competent modified vaccinia virus Tiantan (MVTT) is an attractive vaccine vector, yet little is known about its tissue tropism and pathology in vivo. Recently, we demonstrated that a recombinant MVTT expressing the spike glycoprotein of SARS-CoV (namely MVTT-S) is superior to the non-replicating modified vaccinia Ankara (MVA-S) for inducing high level of neutralizing antibodies through mucosal vaccination. In this study, we further determined the tissue tropism and safety of MVTT-S after the vaccine was administrated through various routes including: intramuscular (i.m.), intranasal (i.n.), and intravaginal (i.vag.) inoculations, respectively. Using real-time PCR, nested PCR, immunohistochemistry and in situ hybridization assays, we found that MVTT-S was able to produce a transient infection in all cases within 48 hr post-inoculation, yet the major site of viral replication in various tissues or organs was dependent on the route of viral administration. We demonstrated that i.m. injection of MVTT-S primarily targeted draining inguinal lymph nodes, whereas mucosal inoculation had broader range of tissue infections. i.n. inoculation involved infections in lungs, kidneys, spleens and cervix lymph nodes while i.vag. administration targeted uteruses, ovaries, kidneys and spleens. Critically, the infection did not cause severe pathogenic consequences in infected tissues, which was consistent to the attenuated phenotype of MVTT-S. Our findings have implications for the optimization of vaccination route and for studies on the correlation between the magnitude of immune responses and the extent of tissue involvement in vivo.
DOI: 10.1002/jmv.21667
PubMed: 20336714
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001728
- to stream PubMed, to step Curation: 001728
- to stream PubMed, to step Checkpoint: 001565
- to stream Ncbi, to step Merge: 002101
- to stream Ncbi, to step Curation: 002101
- to stream Ncbi, to step Checkpoint: 002101
- to stream Main, to step Merge: 002450
- to stream Main, to step Curation: 002417
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The route of inoculation determines the tissue tropism of modified vaccinia Tiantan expressing the spike glycoprotein of SARS-CoV in mice.</title><author><name sortKey="Liu, Huan" sort="Liu, Huan" uniqKey="Liu H" first="Huan" last="Liu">Huan Liu</name><affiliation wicri:level="4"><nlm:affiliation>AIDS Center and State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Hubei, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>AIDS Center and State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Hubei</wicri:regionArea><orgName type="university">Université de Wuhan</orgName><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName></affiliation></author><author><name sortKey="Yu, Wenbo" sort="Yu, Wenbo" uniqKey="Yu W" first="Wenbo" last="Yu">Wenbo Yu</name></author><author><name sortKey="Tang, Xian" sort="Tang, Xian" uniqKey="Tang X" first="Xian" last="Tang">Xian Tang</name></author><author><name sortKey="Wang, Haibo" sort="Wang, Haibo" uniqKey="Wang H" first="Haibo" last="Wang">Haibo Wang</name></author><author><name sortKey="Ouyang, Wenjie" sort="Ouyang, Wenjie" uniqKey="Ouyang W" first="Wenjie" last="Ouyang">Wenjie Ouyang</name></author><author><name sortKey="Zhou, Jingying" sort="Zhou, Jingying" uniqKey="Zhou J" first="Jingying" last="Zhou">Jingying Zhou</name></author><author><name sortKey="Chen, Zhiwei" sort="Chen, Zhiwei" uniqKey="Chen Z" first="Zhiwei" last="Chen">Zhiwei Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20336714</idno><idno type="pmid">20336714</idno><idno type="doi">10.1002/jmv.21667</idno><idno type="wicri:Area/PubMed/Corpus">001728</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001728</idno><idno type="wicri:Area/PubMed/Curation">001728</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001728</idno><idno type="wicri:Area/PubMed/Checkpoint">001565</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001565</idno><idno type="wicri:Area/Ncbi/Merge">002101</idno><idno type="wicri:Area/Ncbi/Curation">002101</idno><idno type="wicri:Area/Ncbi/Checkpoint">002101</idno><idno type="wicri:Area/Main/Merge">002450</idno><idno type="wicri:Area/Main/Curation">002417</idno><idno type="wicri:Area/Main/Exploration">002417</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The route of inoculation determines the tissue tropism of modified vaccinia Tiantan expressing the spike glycoprotein of SARS-CoV in mice.</title><author><name sortKey="Liu, Huan" sort="Liu, Huan" uniqKey="Liu H" first="Huan" last="Liu">Huan Liu</name><affiliation wicri:level="4"><nlm:affiliation>AIDS Center and State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Hubei, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>AIDS Center and State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Hubei</wicri:regionArea><orgName type="university">Université de Wuhan</orgName><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName></affiliation></author><author><name sortKey="Yu, Wenbo" sort="Yu, Wenbo" uniqKey="Yu W" first="Wenbo" last="Yu">Wenbo Yu</name></author><author><name sortKey="Tang, Xian" sort="Tang, Xian" uniqKey="Tang X" first="Xian" last="Tang">Xian Tang</name></author><author><name sortKey="Wang, Haibo" sort="Wang, Haibo" uniqKey="Wang H" first="Haibo" last="Wang">Haibo Wang</name></author><author><name sortKey="Ouyang, Wenjie" sort="Ouyang, Wenjie" uniqKey="Ouyang W" first="Wenjie" last="Ouyang">Wenjie Ouyang</name></author><author><name sortKey="Zhou, Jingying" sort="Zhou, Jingying" uniqKey="Zhou J" first="Jingying" last="Zhou">Jingying Zhou</name></author><author><name sortKey="Chen, Zhiwei" sort="Chen, Zhiwei" uniqKey="Chen Z" first="Zhiwei" last="Chen">Zhiwei Chen</name></author></analytic><series><title level="j">Journal of medical virology</title><idno type="eISSN">1096-9071</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Administration, Intranasal</term><term>Administration, Intravaginal</term><term>Animal Structures (pathology)</term><term>Animal Structures (virology)</term><term>Animals</term><term>Immunohistochemistry</term><term>In Situ Hybridization</term><term>Injections, Intramuscular</term><term>Membrane Glycoproteins (genetics)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>Spike Glycoprotein, Coronavirus</term><term>Vaccines, Attenuated (administration & dosage)</term><term>Vaccines, Attenuated (adverse effects)</term><term>Vaccinia virus (genetics)</term><term>Vaccinia virus (physiology)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Tropism</term><term>Viral Vaccines (administration & dosage)</term><term>Viral Vaccines (adverse effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Administration par voie nasale</term><term>Administration par voie vaginale</term><term>Animaux</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Hybridation in situ</term><term>Immunohistochimie</term><term>Injections musculaires</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Réaction de polymérisation en chaîne</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Structures anatomiques de l'animal (anatomopathologie)</term><term>Structures anatomiques de l'animal (virologie)</term><term>Tropisme viral</term><term>Vaccins antiviraux (administration et posologie)</term><term>Vaccins antiviraux (effets indésirables)</term><term>Vaccins atténués (administration et posologie)</term><term>Vaccins atténués (effets indésirables)</term><term>Virus de la vaccine (génétique)</term><term>Virus de la vaccine (physiologie)</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Vaccines, Attenuated</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="adverse effects" xml:lang="en"><term>Vaccines, Attenuated</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Vaccins antiviraux</term><term>Vaccins atténués</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Structures anatomiques de l'animal</term></keywords><keywords scheme="MESH" qualifier="effets indésirables" xml:lang="fr"><term>Vaccins antiviraux</term><term>Vaccins atténués</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term><term>Vaccinia virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Virus de la vaccine</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Animal Structures</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de la vaccine</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Vaccinia virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Structures anatomiques de l'animal</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Animal Structures</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Administration, Intranasal</term><term>Administration, Intravaginal</term><term>Animals</term><term>Immunohistochemistry</term><term>In Situ Hybridization</term><term>Injections, Intramuscular</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Polymerase Chain Reaction</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Tropism</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Administration par voie nasale</term><term>Administration par voie vaginale</term><term>Animaux</term><term>Glycoprotéine de spicule des coronavirus</term><term>Hybridation in situ</term><term>Immunohistochimie</term><term>Injections musculaires</term><term>Réaction de polymérisation en chaîne</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Tropisme viral</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The live replication-competent modified vaccinia virus Tiantan (MVTT) is an attractive vaccine vector, yet little is known about its tissue tropism and pathology in vivo. Recently, we demonstrated that a recombinant MVTT expressing the spike glycoprotein of SARS-CoV (namely MVTT-S) is superior to the non-replicating modified vaccinia Ankara (MVA-S) for inducing high level of neutralizing antibodies through mucosal vaccination. In this study, we further determined the tissue tropism and safety of MVTT-S after the vaccine was administrated through various routes including: intramuscular (i.m.), intranasal (i.n.), and intravaginal (i.vag.) inoculations, respectively. Using real-time PCR, nested PCR, immunohistochemistry and in situ hybridization assays, we found that MVTT-S was able to produce a transient infection in all cases within 48 hr post-inoculation, yet the major site of viral replication in various tissues or organs was dependent on the route of viral administration. We demonstrated that i.m. injection of MVTT-S primarily targeted draining inguinal lymph nodes, whereas mucosal inoculation had broader range of tissue infections. i.n. inoculation involved infections in lungs, kidneys, spleens and cervix lymph nodes while i.vag. administration targeted uteruses, ovaries, kidneys and spleens. Critically, the infection did not cause severe pathogenic consequences in infected tissues, which was consistent to the attenuated phenotype of MVTT-S. Our findings have implications for the optimization of vaccination route and for studies on the correlation between the magnitude of immune responses and the extent of tissue involvement in vivo.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><noCountry><name sortKey="Chen, Zhiwei" sort="Chen, Zhiwei" uniqKey="Chen Z" first="Zhiwei" last="Chen">Zhiwei Chen</name><name sortKey="Ouyang, Wenjie" sort="Ouyang, Wenjie" uniqKey="Ouyang W" first="Wenjie" last="Ouyang">Wenjie Ouyang</name><name sortKey="Tang, Xian" sort="Tang, Xian" uniqKey="Tang X" first="Xian" last="Tang">Xian Tang</name><name sortKey="Wang, Haibo" sort="Wang, Haibo" uniqKey="Wang H" first="Haibo" last="Wang">Haibo Wang</name><name sortKey="Yu, Wenbo" sort="Yu, Wenbo" uniqKey="Yu W" first="Wenbo" last="Yu">Wenbo Yu</name><name sortKey="Zhou, Jingying" sort="Zhou, Jingying" uniqKey="Zhou J" first="Jingying" last="Zhou">Jingying Zhou</name></noCountry><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Liu, Huan" sort="Liu, Huan" uniqKey="Liu H" first="Huan" last="Liu">Huan Liu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002417 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002417 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:20336714
|texte= The route of inoculation determines the tissue tropism of modified vaccinia Tiantan expressing the spike glycoprotein of SARS-CoV in mice.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20336714" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |